Abstract : Indonesian leaffish (Pristolepis grootii) is one of Indonesia's endemic fish species that has the potential to be cultivated. The unavailability of quality seeds is a main obstacle in P. grooti farming. This study aimed to induce gonadal maturation of P. grootii. A total of 95 females (100.81 ± 0.78 g), reared in 15 aquaria (n=9), fed with a commercial pellet, and injected twice on the 7th and 14th day.with PMSG 10 IU.kg-1 of BW (T1), PMSG 10 + LHRHa 50 g.kg-1 of BW (T2), PMSG 20 (T3), PMSG 20 +